Misfolding of the Azoarcus group I intron ribozyme 1 The Azoarcus Group I Intron Ribozyme Misfolds and Is Accelerated for Refolding by ATP-dependent RNA Chaperone Proteins*
نویسندگان
چکیده
Background: Group I introns are valuable for studying RNA folding and chaperone proteins. Results: A catalytic activity assay was developed and used to demonstrate two prominent phases for Azoarcus ribozyme folding. The slow phase displays hallmarks of a misfolded intermediate. Conclusion: This RNA accumulates a misfolded intermediate and interacts productively with RNA chaperones. Significance: Delineating misfolding and chaperone roles is crucial for understanding how cellular RNAs fold.
منابع مشابه
The Azoarcus group I intron ribozyme misfolds and is accelerated for refolding by ATP-dependent RNA chaperone proteins.
Structured RNAs traverse complex energy landscapes that include valleys representing misfolded intermediates. In Neurospora crassa and Saccharomyces cerevisiae, efficient splicing of mitochondrial group I and II introns requires the DEAD box proteins CYT-19 and Mss116p, respectively, which promote folding transitions and function as general RNA chaperones. To test the generality of RNA misfoldi...
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